An-Najah University Journal for Research - A (Natural Sciences)

Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine

Article info

2015-04-15
2015-12-21
2016-03-03
127 - 140

Keywords

  • Palestine;
  • Potato
  • PVX.
  • virus;
  • RT-PCR;
  • PVY;

Abstract

Sanitary status of potatoes grown in Northern fields of Palestine; based on their viral infections, was assessed biologically and molecularly in this study. Visual inspections for symptomatic disorders were carried during the growing seasons of 2013-2014, reporting a leaf curling, yellowing and stunting plant syndromes. Molecularly, two of most economically damaging viruses (Potato virus Y and Potato virus X) were detected by reverse transcriptase polymerase chain reaction (RT-PCR), with total infection (9.5%). PVY but not PVX, was found in several locations and cultivars. Beside sanitary assessment of potato, this is the first to report the detection of both potato viruses (PVX and PVY) molecularly in Palestinian fields.

Recommended Citation

Alkowni, R., & Abu-Qaoud, H. (2016). Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine. An-Najah University Journal for Research - A (Natural Sciences), 30(1), 127–140. https://doi.org/10.35552/anujr.a.30.1.1178
[1]R. Alkowni and H. Abu-Qaoud, “Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine,” An-Najah University Journal for Research - A (Natural Sciences), vol. 30, no. 1, pp. 127–140, Feb. 2016, doi: 10.35552/anujr.a.30.1.1178.
Alkowni, Raed, and Hassan Abu-Qaoud. “Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine.” An-Najah University Journal for Research - A (Natural Sciences), vol. 30, no. 1, Feb. 2016, pp. 127–40. Crossref, https://doi.org/10.35552/anujr.a.30.1.1178.
1.Alkowni R, Abu-Qaoud H. Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine. An-Najah University Journal for Research - A (Natural Sciences) [Internet]. 2016 Feb;30(1):127–40. Available from: http://dx.doi.org/10.35552/anujr.a.30.1.1178
Alkowni, Raed, and Hassan Abu-Qaoud. “Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine.” An-Najah University Journal for Research - A (Natural Sciences) 30, no. 1 (February 2016): 127–40. https://doi.org/10.35552/anujr.a.30.1.1178.

مسح ميداني وكشف جزيئي لفيروسات البطاطا في حقول الشمال من فلسطين

معلومات المقال

2015-04-15
2015-12-21
2016-03-03
127 - 140

الكلمات الإفتتاحية

  • Palestine;
  • Potato
  • PVX.
  • virus;
  • RT-PCR;
  • PVY;

الملخص

في هذه الدراسة واستنادا إلى قياس نسب الاصابة بالفيروسات، جرى اول تقييم بيولوجي وجزيئي للوضع الصحي لمحصول البطاطا المزروعة في حقول الشمال من فلسطين، حيث اجريت عدة زيارات حقلية للكشف عن اية اعراض مرضية على المحصول خلال مواسم النمو من 2013-2014، وقد لوحظ وجود اعراض امراض فيروسية مثل التفاف الورق، والاصفرار، واعراض تقزم النبات. جزيئيا، تم الكشف عن اثنين من أكثر الفيروسات الضارة اقتصاديا (فيروس البطاطس Y وفيروس البطاطا X) من خلال فحص مبلمرة سلسلة الحمض النووي باستخدام تقنية(RT-PCR)، لتظهر نسبة الإصابة بالفيروسات (9.5٪). وقد تم العثور على فيروس البطاطس Y في عدة مواقع وأصناف بعكس فيروس البطاطا X الذي انحصر في صنف واحد هو (الاسكا). تعتبر هذه الدراسة الاولى في فلسطين للإبلاغ عن الكشف عن تلك الفيروسات من خلال الفحص الجزيئي، بجانب كونها اول عملية تقييم لصحية نبات البطاطا، المزروع في حقول شمال فلسطين.

Recommended Citation

Alkowni, R., & Abu-Qaoud, H. (2016). Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine. An-Najah University Journal for Research - A (Natural Sciences), 30(1), 127–140. https://doi.org/10.35552/anujr.a.30.1.1178
[1]R. Alkowni and H. Abu-Qaoud, “Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine,” An-Najah University Journal for Research - A (Natural Sciences), vol. 30, no. 1, pp. 127–140, Feb. 2016, doi: 10.35552/anujr.a.30.1.1178.
Alkowni, Raed, and Hassan Abu-Qaoud. “Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine.” An-Najah University Journal for Research - A (Natural Sciences), vol. 30, no. 1, Feb. 2016, pp. 127–40. Crossref, https://doi.org/10.35552/anujr.a.30.1.1178.
1.Alkowni R, Abu-Qaoud H. Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine. An-Najah University Journal for Research - A (Natural Sciences) [Internet]. 2016 Feb;30(1):127–40. Available from: http://dx.doi.org/10.35552/anujr.a.30.1.1178
Alkowni, Raed, and Hassan Abu-Qaoud. “Field Survey and Molecular Detection of Potato Viruses in Northern Fields of Palestine.” An-Najah University Journal for Research - A (Natural Sciences) 30, no. 1 (February 2016): 127–40. https://doi.org/10.35552/anujr.a.30.1.1178.

Why should you
Publish With Us?
An-Najah National University
Nablus, Palestine
P.O. Box
7, 707
Fax
(970)(9)2345982
Tel.
(970)(9)2345560
(970)(9)2345113/5/6/7-Ext. 2628
E-mail
[email protected]
EIC
Prof. Waleed Sweileh

An-Najah University Journal for Research - A (Natural Sciences) by An-Najah University, Nablus, Palestine is licensed under CC BY-NC 4.0