An-Najah University Journal for Research - A (Natural Sciences)

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Keywords

• Antioxidant;
• α-Amylase
• α-Glucosidase;
• multicaulis;
• Antilipase;
• Stiefia

Abstract

Background: Herbal products encompass a wide range of natural substances that show great potential as therapeutic materials. Consequently, there has been a substantial investigation into the therapeutic potential of numerous plant species in recent times. The present study sought to determine the components and assess the antioxidant, antilipase, α-amylase, and α-glycosidase enzyme inhibitory activities of Stiefia multicaulis (Vahl) Soják Vahl hexane, acetone, methanol, and aqueous fractions. Methods: Standard pharmacopeia methods were used to conduct screenings for both quantitative and qualitative phytoconstituents. The lipase, α-amylase, and α-glycosidase enzyme inhibitory activities were assessed using established reference methods. In addition, the antioxidant activity was assessed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: The screening methods of pharmacopeias revealed that S. multicaulis contains a wide range of secondary metabolites, especially tannins. However, the most potent antioxidant, antilipase, α-glucosidase, and α-amylase inhibitory activities were acetone fraction, hexane, methanol, and aqueous S. multicaulis fractions, respectively. Conclusion: The current study outcomes are the first on the α-amylase, lipase, α-glucosidase inhibitory activities of S. multicaulis four solvent fractions. Further investigations are needed to assess the in vivo potential of these plant fractions in animal models.

معلومات المقال

قيد النشر

الكلمات الإفتتاحية

• Antioxidant;
• α-Amylase
• α-Glucosidase;
• multicaulis;
• Antilipase;
• Stiefia

الملخص

Background: Herbal products encompass a wide range of natural substances that show great potential as therapeutic materials. Consequently, there has been a substantial investigation into the therapeutic potential of numerous plant species in recent times. The present study sought to determine the components and assess the antioxidant, antilipase, α-amylase, and α-glycosidase enzyme inhibitory activities of Stiefia multicaulis (Vahl) Soják Vahl hexane, acetone, methanol, and aqueous fractions. Methods: Standard pharmacopeia methods were used to conduct screenings for both quantitative and qualitative phytoconstituents. The lipase, α-amylase, and α-glycosidase enzyme inhibitory activities were assessed using established reference methods. In addition, the antioxidant activity was assessed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: The screening methods of pharmacopeias revealed that S. multicaulis contains a wide range of secondary metabolites, especially tannins. However, the most potent antioxidant, antilipase, α-glucosidase, and α-amylase inhibitory activities were acetone fraction, hexane, methanol, and aqueous S. multicaulis fractions, respectively. Conclusion: The current study outcomes are the first on the α-amylase, lipase, α-glucosidase inhibitory activities of S. multicaulis four solvent fractions. Further investigations are needed to assess the in vivo potential of these plant fractions in animal models.