This study addressed the recycling of animal manure and the use of renewable energy sources through the safe and efficient application of organic fertilizers. The aim was to obtain and identify methane-producing bacteria and evaluate their effectiveness as biological starters in biogas production. Methanobacterium sp. was isolated from poultry manure, cow manure, and wastewater, and identified using morphological and biochemical methods. It was then cultivated for use as a starter in fermentation. Poultry manure was selected as the substrate, with four treatments applied: a control without starter, and three treatments with starters derived from poultry manure, cow manure, and wastewater. Results showed significant differences in bacterial abundance, with cow manure containing the highest count, at 11.52 Log (cfu/g), followed by wastewater, at 8.32 Log (cfu/g), and poultry manure, at 5.19 Log (cfu/g). Gas production indicators further confirmed that the cow manure starter (T3) achieved the highest methane yield, reaching 166 ppm on day 11, followed by the wastewater starter (T4, 72 ppm), the poultry starter (T2, 110 ppm), and the control (50 ppm). Based on these findings, a digital biofermenter (T5) was developed and tested, demonstrating superior efficiency by producing 185 ppm on day 9, which was 65 ppm higher than T3 at the same time and two days earlier. In conclusion, cow manure was identified as the most effective source of methanogenic bacteria, and the developed biofermenter significantly improved both gas yield and fermentation time, providing a practical innovation for clean energy production and environmental sustainability.

This study addressed the recycling of animal manure and the use of renewable energy sources through the safe and efficient application of organic fertilizers. The aim was to obtain and identify methane-producing bacteria and evaluate their effectiveness as biological starters in biogas production. Methanobacterium sp. was isolated from poultry manure, cow manure, and wastewater, and identified using morphological and biochemical methods. It was then cultivated for use as a starter in fermentation. Poultry manure was selected as the substrate, with four treatments applied: a control without starter, and three treatments with starters derived from poultry manure, cow manure, and wastewater. Results showed significant differences in bacterial abundance, with cow manure containing the highest count, at 11.52 Log (cfu/g), followed by wastewater, at 8.32 Log (cfu/g), and poultry manure, at 5.19 Log (cfu/g). Gas production indicators further confirmed that the cow manure starter (T3) achieved the highest methane yield, reaching 166 ppm on day 11, followed by the wastewater starter (T4, 72 ppm), the poultry starter (T2, 110 ppm), and the control (50 ppm). Based on these findings, a digital biofermenter (T5) was developed and tested, demonstrating superior efficiency by producing 185 ppm on day 9, which was 65 ppm higher than T3 at the same time and two days earlier. In conclusion, cow manure was identified as the most effective source of methanogenic bacteria, and the developed biofermenter significantly improved both gas yield and fermentation time, providing a practical innovation for clean energy production and environmental sustainability.